What is a tissue culture
The propagation of a plant by using a
plant part or single cell or group cell in a test tube under very
controlled and hygienic conditions is called "Tissue Culture".
Cultures or plant material is obtained from
customer and mass multiplied in the laboratory. This process is called
Micro-propogation which comes under Biotechnology. The whole process is as
- Incoming Culture/Plant
- Clean material is taken for cutting
under sterile bench in new media
- Preparation of sterile media
- Inoculated material is kept in the
growth rooms in controlled conditions of light and temperature
- Checking for contamination
- Taken to the Dissection Area for
Cutting and multiplying the plant
- Growth Room
- Put for Rooting
Advantages of tissue
- Large number of plants is produced in
a short period.
- It is not seasonal dependant.
- It can produce disease free plants.
- The production of exact copies of
plants that produce particularly good flowers, fruits, or have other
- The production of multiples of plants
in the absence of seeds or necessary pollinators to produce seeds.
- The regeneration of whole plants from
plant cells that have been genetically modified.
- The production of plants in sterile
containers that allows them to be moved with greatly reduced chances of
transmitting diseases, pests, and pathogens.
- The production of plants from seeds
that otherwise have very low chances of germinating and growing, i.e.:
orchids and nepenthes.
- To clean particular plant of viral and
other infections and to quickly multiply these plants as 'cleaned stock'
for horticulture and agriculture.
Disadvantages of tissue
- Technical skill is needed and is
- Cost of propagule is very high.
Important tissue culture
- Grow more biotech Ltd
- AVT Biotechnology
- AG Bioteck
- Calcutta tissue culture products Pvt. Ltd
- Hindustan Agri-genentics Limited