Tissue Culture


What is a tissue culture

The propagation of a plant by using a plant part or single cell or group cell in a test tube under very controlled and hygienic conditions is called "Tissue Culture".

Process description Cultures or plant material is obtained from customer and mass multiplied in the laboratory. This process is called Micro-propogation which comes under Biotechnology. The whole process is as follows:
  • Incoming Culture/Plant
  • Clean material is taken for cutting under sterile bench in new media
  • Preparation of sterile media
  • Inoculated material is kept in the growth rooms in controlled conditions of light and temperature
  • Checking for contamination
  • Taken to the Dissection Area for Cutting and multiplying the plant
  • Growth Room
  • Put for Rooting
  • Dispatching
Advantages of tissue culture
  • Large number of plants is produced in a short period.
  • It is not seasonal dependant.
  • It can produce disease free plants.
  • The production of exact copies of plants that produce particularly good flowers, fruits, or have other desirable traits.
  • The production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds.
  • The regeneration of whole plants from plant cells that have been genetically modified.
  • The production of plants in sterile containers that allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens.
  • The production of plants from seeds that otherwise have very low chances of germinating and growing, i.e.: orchids and nepenthes.
  • To clean particular plant of viral and other infections and to quickly multiply these plants as 'cleaned stock' for horticulture and agriculture.
Disadvantages of tissue culture
  • Technical skill is needed and is expensive.
  • Cost of propagule is very high.
Important tissue culture labs
  • Grow more biotech Ltd
  • AVT Biotechnology
  • AG Bioteck
  • Calcutta tissue culture products Pvt. Ltd
  • Hindustan Agri-genentics Limited
Reference http://www.jains.com/Tissue/tissueculture.htm